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For buffer use in HPLC, I have collected data on the pKa,
maximum buffer working range and UV cutoff. For an extended list of
buffer ranges but rounded at 0.5 pH units, see the pKa/pKb
tabel elsewhere on this site.
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| buffer |
pKa (25°C) |
max.buffer range |
UV cutoff (nm) |
| TFA |
0,3 |
|
210 (0.1%) |
| phosphate, pKa1 |
2,1 |
1.1-3.1 |
< 200 |
| phosphate, pKa2 |
7,2 |
6.2-8.2 |
< 200 |
| phosphate, pKa3 |
12,3 |
11.3-13.3 |
< 200 |
| citrate, pKa1 |
3,1 |
2.1-4.1 |
230 |
| citrate, pKa2 |
4,7 |
3.7-5.7 |
230 |
| citrate, pKa3 |
6,4 |
5.4-7.4 |
230 |
| carbonate, pKa1 |
6,1 |
5.1-7.1 |
< 200 |
| carbonate, pKa2 |
10,3 |
9.3-11.3 |
< 200 |
| formate |
3,8 |
2.8-4.8 |
210 (10 mM) |
| acetate |
4,8 |
3.8-5.8 |
210 (10 mM) |
| ammonia |
9,3 |
8.3-10.3 |
200 (10 mM) |
| borate |
9,2 |
8.2-10.2 |
n/a |
| TEA |
10,8 |
9.8-11.8 |
< 200 |
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More links...
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- A very good site about buffer preparation where
you can even enter the ionic concentration, is here.
- Alternative buffer preparation from ZirChrom.
- Enter a pH and get back the right buffer at Reall.
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Updates & Comments
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Please use this
form to notify me if you have any more data on the preparation of
buffers.
http://www.raell.demon.co.uk/chem/calcs/LC_calc/index.htm
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