What's on this page?
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| This HPLC column selection chart will help you choose the right HPLC
column for your analysis or application. Simply determine the molecular weight
of your analyte, and follow the chart downwards. The best suited HPLC
separation mode and packing material is
at the bottom of these charts. |
Column selection chart:
molecular weight < 2000 g/mol
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|
water soluble |
organic solvent soluble |
| non-ionic |
ionic |
THF soluble |
hexane soluble |
methanol and methanol/water soluble |
| LC mode
(packing) |
RP bonded (phenyl, CN, C8, C18) |
RP ionization control
(phenyl, CN, C8, C18) |
RP polymeric |
RP paired-ion |
ion exchange (SAX, SCX) |
ion chromato-
graphy |
small molecule size exclusion |
NP adsorbtion (Si) |
NP bonded (CN, NH2) |
RP bonded( CN, NH2, phenyl C8, C18) |
Column selection chart: molecular weight
> 2000 g/mol
|
|
water soluble |
organic solvent soluble |
| LC mode (packing) |
RP bonded (C18, phenyl, C4) |
aqeous gel filtration
(SEC) |
ion exchange |
hydrophobic interaction |
affinity |
size exclusion chromatography |
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