Typical Column Properties

What's on this page?

In the table below, typical properties like flow, pressure, sample load and sample volume of columns of different diameter, length and particle diameter can be found. These values can be used to estimimate scaling factor for scaling your HPLC method. To calculate the void volume of a HPLC column, use the void volume calculator below.

Void volume

Enter the diameter and length and click 'calculate' to calculate the column void volume. The pore volume of 0.7 is a good average for HPLC columns, but you can enter a different value for this. For the calculation, the following formula is used:


diameter (mm)
length (mm)
average pore volume
void volume (ml)

On Chiralizer.com you can find a table with pre-calculated HPLC column void values, but if your column is not in the list, use the calculator on this page.

HPLC column properties

dp (µm)	ID (mm)	L (mm)	typical flow (mL/min)	typical pressure*		N	t_o (mL)	sample load (mg)**	sample volume (µL)
dp (µm)	ID (mm)	L (mm)	typical flow (mL/min)	(psi)	(bar)	N	t_o (mL)	sample load (mg)**	sample volume (µL)
5	1	150	0.1	1500	103.4	-	-	~0.05	0.05-1
5	2	150	0.2	750	51.7	-	-	-	-
3	3	150	0.42	1586	109.4	12500	0.85	--	-
4	3	150	0.42	892	61.5	9375	0.85	--	-
5	3	150	0.42	571	39.4	7500	0.85	--	-
5	3.2	150	0.48	571	39.4	7500	0.97	--	-
3	4.6	150	0.5	985	68.0	-	1.99	-	-
5	4.6	150	1	571	39.4	7500	1.99	--	-
10	4.6	150	2	355	24.5	-	1.99	-	-
5	10	150	5	750	51.7	-	-	-	-
10	21.2	150	20	170	11.7	-	-	-	-
5	1	250	0.1	2500	172.4	-	-	-	-
5	2	250	0.2	1250	86.2	-	-	0.2	0.2-5
5	3	250	0.42	952	65.6	12500	1.41	--	-
5	3.2	250	0.48	952	65.6	12500	1.61	1	1-20
3	4.6	250	0.5	1640	113.1	-	3.32	-	-
5	4.6	250	1	1180	81.4	12500	3.32	5	5-80
10	4.6	250	2	590	40.7	-	-	-	-
5	10	250	5	1250	86.2	-	9.82	30	30-500
10	21.2	250	20	280	19.3	-	-	-	-

* typical values for water as eluens
** dependant on sample composition
A handy tool for scaling your HPLC method is offered at Reall

last update: augustus 11, 2007